Hcs lipidtox
WebOct 30, 2024 · HCS LipidTOX™ Phospholipidosis and Steatosis detection kits; The kit has following components (a) Red phospholipidosis detection reagent (1000× stock). (b) … Webthe Invitrogen ™ HCS LipidTOX neutral lipid stains were developed to detect intracellular lipid droplets or globules and characterize the effects of drugs and other compounds on lipid metabolism in mammalian cell lines. These reagents are added after cell fixation (Figure 5), Figure 2. Real-time monitoring of the formation of OxLDL.
Hcs lipidtox
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WebDec 7, 2024 · Lastly, we used high-content screening (HCS) to determine the levels of reactive oxygen species (ROS), lipid, and mitochondrial membrane potential (MMP). Results: The ethanol extraction process aggravated the hepatotoxicity of … WebInvitrogen™ HCS LipidTOX™ Green Neutral Lipid Stain, for cellular imaging. High affinity for neutral lipid droplets. Brand: Invitrogen™ H34475. Code : 50. Additional Details : …
WebHCS LipidTOX™ phospholipidosis detec-tion reagents can be easily detected with fluorescence microscopes or HCS readers equipped with standard filter sets (Figure … WebHCS LipidTOX™ Deep Red, 1000 X. Names; Short Name: HCS LipidTOX™ Deep Red. Synonym(s) HCS LipidTOX™ Deep Red. InChI Key (linked to PubChem) N/A. …
WebDec 2, 2024 · Adipocyte lipid droplet staining was performed with HCS LipidTOX™ Deep Red Neutral Lipid Stain (Thermo Fisher, Waltham, MA, USA). After washing cells with PBS three times, the adipocytes were fixed with 4% formaldehyde and incubated for 30 min at room temperature. Next, the formaldehyde was removed, and the cells were gently … WebHCS LipidTOX Phospholipidosis and Steatosis Detection Kit offers a complete set of reagents for performing image-based high-content screening (HCS) assays to characterize phospolipidosis and steatosis, toxic side effects on lipid metabolism that can be triggered by drugs or other compounds. The kit supplies LipidTOX Red phospholipid stain and ...
WebAug 20, 2024 · To better characterize a potential role of TMEM41B in regulating LDs, we analyzed H4 cells basally or upon treatment with BSA-conjugated oleic acid by probing with HCS LipidTox Green Neutral Lipid Stain, a probe with high affinity for neutral LDs.
WebExcess lipid accumulates in vesicles that displace the cytoplasm; large accumulations of lipids can disrupt cell constituents, and in severe cases, the cell may even burst. Steatosis is a common consequence of hepatocellular insult, which can be drug-induced or can be an outcome in pathophysiogical disease progression. hewan berikut yang bergerak dengan kaki semu (pseudopodia) adalahWebHCS LipidTOX™ Neutral Lipid Stains 2 The LipidTOX™ neutral lipid stains are designed for fixed–end point workflows in which form-aldehyde-fixed cells in microplates are … hewan berkaki 5WebHCS LipidTOX Green Neutral Lipid is a fluorescent compound with an excitation peak at 498 nm and an emission peak at 507 nm. It can be excited using a 488 nm laser paired … ezabel mulhouseWebHCS LipidTOX Phospholipidosis and Steatosis Detection Kit offers a complete set of reagents for performing image-based high-content screening (HCS) assays to … ezabella martellaWebMar 28, 2015 · HCS LipidTOX™ Red Neutral Lipid stain has an extremely high affinity for neutral lipid droplets and is best imaged with filter sets appropriate for Alexa Fluor® 594 dye or Texas Red® dye (ex577-em609 nm). The XY coordinates were annotated in order to recognize the same microscopic field. Treble staining allowed us to evaluate possible ... ezabehWebHcs Lipidtox Deep Red Neutral Lipid Stain, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - … hewan berkaki banyak ttsWebDec 17, 2016 · After 48 culture140 Glocket al. Vitro35 (2016) 139–148 mediumwas replaced freshmedium, including HCS LipidTOX Green phospholipidosis detection reagent (LipidTOX) (Invitrogen, Carlsbad, US) (1:1000), respectiveconcentration bina-ry combinations Samplescontaining LipidTOX respectivesolvent(s) (vehicle controls) were … hewan berkaki 2 kartun