Leaf disk assay experiment

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August undefined, 2024

WebLeaf disk transformation of tobacco is a very simple and robust method. It is used with success in many laboratories. The protocol presented here is a simplified version of that … Webprobably due to the increased leaf area and therefore increased surface area for light absorption. Temperature of the room The experiment will be carried out in a darkened room. The main experiment will be carried in one series of data collections therefore the temperature of the room should remain constant. This will be monitored by

Photosynthesis in Leaf Discs - University of Washington

Web17 jul. 2013 · Tap the syringe to suspend the leaf disks in the solution. Holding a finger over the syringe opening side, draw back on the plunger (~ 1 mL) to create a vacuum. Hold this vacuum for about 10 seconds. While holding the vacuum, swirl the leaf disks to suspend them in the solution. Let’s off the vacuum. WebInfiltrate the leaf disks with sodium bicarbonate solution. Remove the piston or plunger and place the leaf disks into the syringe barrel. Replace the plunger being careful not to … erik the red\u0027s language

Lab: Photosynthesis - WikiEducator

WebFloating Leaf Discs in a Vacuum Expand NOTE: In this experiment you will indirectly observe photosynthesis and cellular respiration using a floating leaf disc in a solution. During photosynthesis, air bubbles will cause the … Web17 okt. 2024 · The leaf disk assay measures the rate of oxygen production in the leaf disks, which is a representation for the rate of photosynthesis. When you placed … WebThis was done until all the leaf disks sunk in the solution and were ready for testing. The leaf disks and solution were then put back in the cup. The cup was placed under the light and the number of leaf disks floating were counted and recorded at one minute intervals for 25 minutes. Once all the disks were floating or when 25 minutes were over, find the zeros and their multiplicities

Effect of altitude and solvent on Psidium guajava Linn. leaves …

Floating Leaf Disks Lab (Photosynthesis) - YouTube

WebEDVOTEK® The Biotechnology Education Company® Web22 feb. 2013 · Banana bunchy top virus (BBTV) is the most destructive pathogenic virus of banana plants worldwide. The virus is transmitted in a circulative non-propagative manner by the banana aphid, Pentalonia nigronervosa Coquerel. In this work, we examined the localization, accumulation, and transmission efficiency of BBTV in four laboratory … erik the red parents find the zeros by completing the square

"WebThis is your control. For your experimental disks, place the cover of the Petri dish facing upwards on top of the Petri dish with solution and ... an assay utilizing leaf disks. Am. Bio. Teacher 47(2): 96-99. Materials List Plants: fresh leaves from rapidly growing plants (no geraniums or succulents); Swiss chard, spinach, beans (English ... " - Leaf disk assay experiment

Leaf disk assay experiment

The Floating Disk Assay to Study Photosynthesis–an update

Web21 sep. 2012 · Transient Agrobacterium-mediated gene expression assays for Nicotiana tabacum (N. tabacum) are frequently used because they facilitate the comparison of multiple expression constructs regarding their capacity for maximum recombinant protein production. However, for three model proteins, we found that recombinant protein accumulation (rpa) … Web13 feb. 2024 · The leaf discs from spinach, kale, collards, mustard, and watermelon were used to optimize the reagent concentration and reaction time for each assay. For the ABTS leaf disc assay, the...

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Weball leaf disks are illuminated uniformly and illumination is always normal to the plane of the leaf disks. Since the leaf disks are at a fixed distance from the light source, we have varied the light intensity by in-terposing squares (5 inches by 5 - 4 - Figure 1. Sketch of the experimental appa-ratus for photosynthesis assay. The dis- WebTurn on a bright light, and monitor the disks every minute. Count how many disks are floating during each of the next 15 minutes. After all (or most) of the disks are floating, put the cups in the dark (a shoebox or a closet) and monitor for the next 15 minutes.

WebBackground for Experimental Procedure: Leaf disks from dicot leaves (use a hole puncher and ‘punch’ a leaf = leaf disk) float, normally. When the air spaces are infiltrated (filled … WebSodium hydrogen bicarbonate was used in this experiment to supply carbon dioxide to the pineapple leaf discs to perform photosynthesis. The water does not have a high concentration of carbon dioxide dissolved in it but the amount is sufficient for the aquatic plants to survive. Hence, by using sodium hydrogen bicarbonate will increase the ...

WebThe soap ‘wets’ the hydrophobic surface of the leaf allowing the solution to be drawn into the leaf. 3. Cut 10 or more uniform leaf disks. Use the single-hole punch at your bench. Avoid... Web7. Explain the changes that occurred within the leaf tissue that allowed the leaf discs to rise to the surface. 8. Imagine that you had 1-2 additional class periods available for follow-up experiments with a setup similar to this one. Propose another experiment that you could do to test the effect of another factor (other than light) on ...

WebKeywords Black leaf streak disease Leaf disk assay Musa spp. Mycosphaerella ﬁjiensis Transgenic banana Introduction Banana is the main fruit in international trade in terms of volume and the fourth most important staple crop with a …

Web2 sep. 2024 · Leaf disks float, normally. When the air spaces are infiltrated with the solution the overall density of the leaf disk increases and the disk sinks. The … find the zeros of a quadratic equationWebPhotosynthesis lab. Purpose: The purpose of this experiment was to determine the effect of sodium bicarbonate on the rate of photosynthesis of spinach leaf disks. Hypothesis: The leaf disks placed in the water solution with sodium bicarbonate will float to the top faster and in a higher quantity because the sodium bicarbonate will serve as a ... erik the red\u0027s daughterWeb12 dec. 2013 · Using a Leaf Disk Assay. Photosynthetic rates can be determined over 20 minutes using a leaf disk assay. After removing the air from a syringe containing the leaflets and water, the leaflets will sink, indicating that they are now deprived of air. Pour the leaflets into a sodium bicarbonate container and record for every two minutes the amount ... erik the red timelineWeb7 dec. 2024 · To determine photosynthesis, an experiment can be performed on a floating leaf disk. Plants use photosynthesis to convert light, water, and carbon dioxide from the air into oxygen and sugar. How quickly plants generate oxygen is also shown, as well as how variables affect photosynthesis. erik the red\u0027s language crosswordWeb13 apr. 2024 · Cytotoxicity assay of guava leaves extracts LC 50 value of methanol, ethanol and water extract of guava leaves was determined using probit regression analysis (Table 10 ). Water extracts from all three locations were found to be non-toxic, in which WGM had the highest LC 50 value (5.6 × 10 4 µg/ml) whereas methanol extract from all three … erik the red statueWebA Leaf Disk Assay for Detection of Resistance of Melons to Sphaerotheca fuliginea Race 1. R. Cohen, Plant Pathologist, Department of Vegetable Crops, Agricultural Research Organization, Newe Ya’ar Experiment Station, P.O. Haifa 31-999, Israel. . Plant Dis. 77:513-517. Accepted for publication 28 December 1992. find the zeros for the functionWeb16 feb. 2024 · Agrobacterium-mediated leaf-disk method was used for stable genetic transformation according to a previously described protocol . ... Three representative lines based on transcript levels of PhOBF1 in the RNAi or overexpression assay were selected for further experiments. erik the red story